Antibody host
N/A
Antibody type
N/A
Antibody conjugate
Biotin
Tissue
control
Group
isotypes
Stock availability
Available
Conjugation
Biotinylated
Category
Isotype Control
Additional isotype
Fab fragment,IgG
Technical datasheet
Contact Gentaur to request the datasheet or ask our specialists for more information.
Notes
The Bovine IgG Fab-Biotin conjugate (non-immune, isotype control) purified is manufactured for Research Use Only or for diagnostics purposes.
Description
Isotype or positive controls by peptides, antibodies and deactivated samples.Complexes, systems, regulators, immune RNAs and non immune controls that can react with a specific antigen epitope.
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent,Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
