Additional isotype
IgM
Antibody host
N/A
Antibody type
N/A
Antibody conjugate
FITC
Tissue
control
Group
isotypes
Stock availability
Available
Category
Isotype Control
Conjugation
Anti-FITC Antibody
Latin name
Oryctolagus cuniculus
Technical datasheet
Contact Gentaur to request the datasheet or ask our specialists for more information.
Notes
The Rabbit IgM-FITC Conjugate (isotype control, non-immune), purified is manufactured for Research Use Only or for diagnostics purposes.
Description
Isotype or positive controls by peptides, antibodies and deactivated samples.Complexes, systems, regulators, immune RNAs and non immune controls that can react with a specific antigen epitope.
About
Rabbits are used for polyclonal antibody production by adi. Rabbit antibodies are very stable and can be stored for several days at room temperature. adi adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Properties
This adi Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. adi FITC is highly sensitive to pH extremes.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen,Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
