UNIProt ID
NA
Target Synonym
NA
Target Species
Mouse
Target Name
LDL-IC
Type
Sandwich
Sensitivity
3.75 ng/mL
Detection Type
Colormetric
Latin name
Mus musculus
Detection Range
6.25~400ng/mL
Tested Sample Types
Serum, Plasma, Cell supernatant
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
Product Name
Mouse LDL-IC(Low Density Lipoprotein Immune Complex) EIA Kit
Description
High and low density proteins are supplied by Elabscience in volumes of 1. Other densities are possible.This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.Complexes, systems, regulators, immune RNAs and non immune controls that can react with a specific antigen epitope.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LDL-IC. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LDL-IC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LDL-IC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LDL-IC. You can calculate the concentration of Mouse LDL-IC in the samples by comparing the OD of the samples to the standard curve.
